Mouse Genetics and Transgenics PDF Download

Author: Ian J. Jackson
Publisher: OUP Oxford
ISBN: 0191565830
Category : Science
Languages : en
Pages : 324

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Book Description
A unique book that integrates knowledge from a wide range of expertise, specifically applied to the mouse, and addressed at a wide audience from those new to the field to experts who want an update on the state of the art. Mouse Genetics and Transgenics: APA covers all aspects of using the mouse as a genetic model organism: care & husbandry; archiving stocks as frozen embryos or sperm; making new mutations by chemical mutagenesis; transgenesis; and gene targetting; mapping mutations and polygenic traits by cytogenetic, genetic, and physical means; and disseminating and researching information via the Internet.

Author: Shree Ram Singh
Publisher: Humana
ISBN: 9781071610077
Category : Science
Languages : en
Pages : 217

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Book Description
This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.

Author: Paul M. Wassarman
Publisher: Academic Press
ISBN: 9780123848826
Category : Medical
Languages : en
Pages : 580

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Book Description
17 years have passed since the first edition of the Guide to Techniques in Mouse Development (volume 225) was published. During this time development of technology used to investigate mouse development has not stood still, with enormous advances occuring in genomics, transgenic and ES cell methodology, and reprogramming, culminating with development of iPS cells. At both the cellular and molecular levels, a great many technological advances have been made that permit investigators to probe ever more deeply into all aspects of mouse development. This completely new version of the Guide has been split into two parts, both highlighting the techonological advances used to study mouse development.

Author:
Publisher:
ISBN:
Category : Mice
Languages : en
Pages :

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Author: Eugene J. Eisen
Publisher: World Scientific
ISBN: 1860945651
Category : Medical
Languages : en
Pages : 364

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Book Description
The sequencing of the mouse genome has placed the mouse front and center as the most important mammalian genetics model. However, no recent volume has detailed the genetic contributions the mouse has made across the spectrum of the life sciences; this book aims to fill that vacuum. Mouse genetics research has made enormous contributions to the understanding of basic genetics, human genetics, and livestock genetics and breeding. The wide-ranging topics in the book include the mouse genome sequencing effort, molecular dissection of quantitative traits, embryo biotechnology, ENU mutagenesis, and genetics of disease resistance, and have been written by experts in their respective fields.

Author: Monica Justice
Publisher: Springer Science & Business Media
ISBN: 1402050577
Category : Science
Languages : en
Pages : 98

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Book Description
The Second Georgia Genetics Symposium was held color. Soon after, he joined the sta? of The Jackson in September 2000, and the development of this Laboratory in Bar Harbor, Maine. book took place over the nearly 4 years that ensued. Much of Bill’s research at the lab was centered During this time, many advances in the Genome around investigating phenotypic variability within Project and mouse mutagenesis were made. In the highly inbred strains, and in that connection he book overview, we discuss the development of the developed the technique of ovarian transplanta- Genome Project (which is the context for the sym- tion (even using embryonic donors) and a genetic posium), the role the mouse was playing at that scheme whereby graft compatibility could be time, how that role has evolved, and how the combined with the ability to distinguish o?spring chapters of the book address issues in mouse func- from donor and regenerated host ovaries. His tional genetics. Many of the chapters in this book work was in?uenced by the second World War, will provide useful resources for years to come. ?rst because The Jackson Laboratory turned into Of greater impact, our keynote speaker, the a production colony for the military, primarily to mutagenesis pioneer William L. (Bill) Russell, produce mice for typhoid testing, and secondly, passed away on July 23, 2003.

Author: Jacqueline N. Crawley
Publisher: John Wiley & Sons
ISBN: 0470119047
Category : Science
Languages : en
Pages : 544

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Book Description
Dr. Jacqueline N. Crawley, author of the First and Second Editions of What’s Wrong with My Mouse? Behavioral Phenotyping of Transgenic and Knockout Mice,continues to field calls and e-mails from molecular geneticists who ask: how do I run behavioral assays to find out what’s wrong with my mouse? Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, the Second Edition includes two new chapters: "Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis. "Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions. The final chapter, "The Next Generation," examines new and emerging technologies. Throughout the book, the use of behavioral testing equipment is illustrated with photographs, diagrams, and representative data. Examples of behavioral tasks successfully applied to transgenic and knockout mouse models are provided, as well as references to the primary literature and step-by-step methods protocols. These features, along with a comprehensive index, listings of database and vendor websites, and an extensive list of references, make this book a valuable and practical resource for students and researchers.

Author: Chikdu Shakti Shivalila
Publisher:
ISBN:
Category :
Languages : en
Pages : 180

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Book Description
The importance and significance of a model organism in biological research cannot be overstated. The mouse in particular has been very useful in understanding questions in many areas of research such as developmental biology, cancer biology, neuroscience and genetics. However, even though the methods to make transgenic mice and gene knockin and knockouts have been successful, they are very inefficient, labor intensive and costly. Therefore, in this thesis we developed a novel methodology to rapidly and efficiently modify the mouse genome. Using CRISPR/Cas9, a novel genome-engineering technology developed from bacteria, we were able to genetically modify mouse embryonic stem cells and make mice that carried genetic modification by zygotic injections. Using CRISPR/Cas9 we were able to make mice in as little as three weeks that contained multiple gene knockouts, single nucleotide modifications, GFP and mCherry reporter alleles, epitope-tagged alleles, and conditional alleles. Another interesting area of research in mouse genetics is epigenetic regulation, specifically how DNA methylation regulates development, gene expression, and cell state. Multiple studies have shown that this epigenetic modification plays an important regulatory role in these processes; however, the technology that has existed so far to investigate DNA methylation has only been able to look at snapshots of methylation patterns in fixed cell populations. In this thesis we have developed a novel technology named Reporter of Genomic Methylation (RGM), which allows for the investigation of methylation dynamics at single cell-resolution in vivo. The RGM technology was developed using a minimal synthetic secondary DMR promoter that drives the expression of a florescent protein. Using CRISPR/Cas9 the RGM reporter can be integrated into any genomic locus where it can report on the methylation state of its surroundings. We further show that the RGM reporter activity reflects the methylation state of non-coding regulatory elements such as promoters and enhancers. Furthermore, we show that the RGM technology allows for the dynamics of methylation and demethylation to be observed at these non-coding loci as cells transition between a pluripotent and differentiated state.

Author: Robert P. Erickson
Publisher: Academic Press
ISBN: 0128240172
Category : Medical
Languages : en
Pages : 344

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Book Description
Twentieth Century Mouse Genetics: A Historical and Scientific Review provides a comprehensive examination of key advances in mouse genetics throughout the 20th century. Here Dr. Robert P. Erickson, a leader in the field, identifies the contributions of historic mouse genetics studies, and how those approaches and early discoveries are still shaping human genetics research and medical genetics today. In addition to historical overviews, the author provides researcher biographies and updates connecting historic research to ongoing advances. Past studies discussed use the T/t complex as an example and include the origins of mouse genetics, the synthesis of genetics and evolution, cytogenetics and gene mapping, population genetics and mutation research, immunogenetics, reproductive genetics, molecular cloning, X-inactivation and epigenetics, sex determination, and pharmacogenetics. Here researchers, students, and clinicians will find fresh inspiration to engage in human genetics research employing mouse models and to translate those findings to clinical practice. • Offers a comprehensive examination of key advances in mouse genetics throughout the 20th century. • Includes updates connecting historic research to ongoing advances. • Authored by a thought-leader in the field.

Author: Jean Louis Guénet
Publisher: Springer
ISBN: 3662442876
Category : Science
Languages : en
Pages : 408

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Book Description
This book, written by experienced geneticists, covers topics ranging from the natural history of the mouse species, its handling and reproduction in the laboratory, and its classical genetics and cytogenetics, to modern issues including the analysis of the transcriptome, the parental imprinting and X-chromosome inactivation. The strategies for creating all sorts of mutations, either by genetic engineering or by using mutagens, are also reviewed and discussed in detail. Finally, a last chapter outlines the methodology used for the analysis of complex or quantitative traits. The authors also discuss the importance of accurate phenotyping, which is now performed in the mouse clinics established worldwide and identify the limits of the mouse model, which under certain circumstances can fail to present the phenotype expected from the cognate condition in the human model. For each chapter an up-to-date list of pertinent references is provided. In short, this book offers an essential resource for all scientists who use or plan to use mice in their research.