Morphological Mouse Phenotyping PDF Download

Author: Jesus Ruberte
Publisher: Elsevier
ISBN: 0128128771
Category : Mice
Languages : en
Pages :

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Author: Jesus Ruberte
Publisher: Academic Press
ISBN: 9780128128053
Category : Medical
Languages : en
Pages : 600

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Book Description
Disease animal models are fundamental in research to improve human health. The success of using genetically engineered mice to evaluate molecular disease hypothesis has encouraged the development of massive global projects making the mouse the most used animal disease model. Laboratory mouse populations are straining the housing capacity of pharmaceutical and biotechnology companies, as well as public research institutions. However, the scientific community lacks sufficient expertise in morphological phenotyping to effectively characterize and validate these animal models. The mouse displays fundamental morphological similarities to humans. However, a mouse is not a man. Here we present a complete and integrative description of mouse normal morphology. This book, basically an atlas, but also containing explanatory diagrams and text, guides the reader through normal mouse anatomy, histology and imaging, and is aimed for mouse researchers as well as veterinarian and human pathologists. More than 2,200 original images showing the anatomy, histology and cellular structure of mouse organs have been specifically produced for this book in the Mouse Imaging Platform (Center for Animal Biotechnology and Gene Therapy, Universitat Autonoma de Barcelona) In addition, correlative X-ray, Computed Tomography, Magnetic Resonance and Ultrasound images complete this integrative vision of the mouse morphology Classical anatomical techniques such as conventional dissection, skeletal preparations, vascular injections, as well as histological, immunohistochemical and electron microscopy techniques have been employed to characterize the mouse morphology "

Author: Jesús Ruberte París
Publisher:
ISBN:
Category :
Languages : es
Pages : 614

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Book Description
Disease animal models are fundamental in research to improve human health. The success of using genetically engineered mice to evaluate molecular disease hypothesis has encouraged the development of massive European and global projects making the mouse the most used animal disease model. Laboratory mouse populations are straining the housing capacity of pharmaceutical and biotechnology companies, as well as public research institutions. However, the scientific community lacks sufficient expertise in morphological phenotyping to effectively characterize and validate these animal models. The mouse displays fundamental morphological similarities to humans. However, a mouse is not a man. Here we present a complete and integrative description of mouse normal morphology. The main characteristics of this book are: • More than 2,200 original images have been specifically produced for this book in the Mouse Imaging Platform (Center for Animal Biotechnology and Gene Therapy, Universitat Autònoma de Barcelona). • These images show the anatomy, histology and cellular structure of mouse organs. • In addition, correlative X-ray, Computed Tomography, Magnetic Resonance and Ultrasound images complete this integrative vision of the mouse morphology. • Classical anatomical techniques such as conventional dissection, skeletal preparations, vascular injections, as well as histological, immunohistochemical and electron microscopy techniques have been employed to characterize the mouse morphology. This book, basically an atlas, but also containing explanatory diagrams and text, guides the reader through normal mouse anatomy, histology and imaging, and is aimed for mouse researchers as well as veterinarian and human pathologists.

Author: Naoyuki Tanimoto
Publisher:
ISBN: 9781493977208
Category : Mice
Languages : en
Pages : 334

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Author: Martin Hrabé de Angelis
Publisher: John Wiley & Sons
ISBN: 3527608702
Category : Science
Languages : en
Pages : 358

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Book Description
This is the first book in the field of mouse genetics to provide comprehensive and standardized methods for the characterization of laboratory mice. The editor is Director of the German Mouse Clinic and member of the Project Committee of the German National Genome Research Network and provides here a brief introduction to the mouse as a model for diseases and functional analysis of genes and proteins. Throughout, he focuses on the characterization of mouse models using the latest phenotyping methods, with the different areas presented in a clearly structured and easily accessible manner.

Author: John P. Sundberg
Publisher: John Wiley & Sons
ISBN: 1119624576
Category : Medical
Languages : en
Pages : 496

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Book Description
An updated and comprehensive reference to pathology in every organ system in genetically modified mice The newly revised and thoroughly updated Second Edition of Pathology of Genetically Engineered and Other Mutant Mice delivers a comprehensive resource for pathologists and biomedical scientists tasked with identifying and understanding pathologic changes in genetically modified mice. The book is organized by body system, includes descriptions and explanations of a wide range of findings, as well as hundreds of color photographs illustrating both common and rare lesions that may be found in genetically engineered and wild type mice. The book is written by experienced veterinary and medical pathologists working in veterinary medical colleges, medical colleges, and research institutes. Covering the latest discoveries in mouse pathology resulting from advancements in biotechnology research over the last 30 years, this singular and accessible resource is a must-read for veterinary and medical pathologists and researchers working with genetically engineered and other mice. Readers will also benefit from: A thorough introduction to mouse pathology and mouse genetic nomenclature, as well as databases useful for analysis of mutant mice An exploration of concepts related to validating animal models, including the Cinderella Effect Practical discussions of basic necropsy methods and grading lesions for computational analyses Concise diagnostic approaches to the respiratory tract, the oral cavity and GI tract, the cardiovascular system, the liver and pancreas, the skeletal system, and other tissues As a one-stop and up to date reference on mouse pathology, Pathology of Genetically Engineered and Other Mutant Mice is an essential book for veterinary and medical pathologists, as well as for scientists, researchers, and toxicologists whose work brings them into contact with genetically modified mice.

Author: Andreas Deutsch
Publisher: Springer Science & Business Media
ISBN: 081764556X
Category : Mathematics
Languages : en
Pages : 386

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Book Description
Volume II of this two-volume, interdisciplinary work is a unified presentation of a broad range of state-of-the-art topics in the rapidly growing field of mathematical modeling in the biological sciences. Highlighted throughout are mathematical and computational apporaches to examine central problems in the life sciences, ranging from the organization principles of individual cells to the dynamics of large populations. The chapters are thematically organized into the following main areas: epidemiology, evolution and ecology, immunology, neural systems and the brain, and innovative mathematical methods and education. The work will be an excellent reference text for a broad audience of researchers, practitioners, and advanced students in this rapidly growing field at the intersection of applied mathematics, experimental biology and medicine, computational biology, biochemistry, computer science, and physics.

Author: Yang Dai (M. Eng.)
Publisher:
ISBN:
Category :
Languages : en
Pages : 54

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Book Description
In this thesis, I developed computational pipelines and algorithms that use high dimensional biomarker imaging data to predict features of tumor tissues taken from a genetically engineered mouse model (GEMM) of lung adenocarcinoma. I extracted biomarker expression levels and morphological, textural, and spatial motifs of single cells from the imaging data and used these features to train algorithms to predict tumor histologic grade, a measure correlated with the malignant potential of a tumor. The algorithm predictions were evaluated through comparison to a validated deep learning model. The random forest algorithm achieved a 72% accuracy classifying cells as belonging to a non-tumor, grade 1, grade 2, or grade 3 region and achieved a 87% accuracy classifying cells as belonging to a tumor or non-tumor region. A combination of biomarker, morphological, textural, and spatial features generated models that performed better than any single group of markers by itself; spatial features in particular significantly improved model performance.

Author: Dennis S. Charney
Publisher: Oxford University Press
ISBN: 0199934959
Category : Medical
Languages : en
Pages : 1222

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Book Description
Our understanding of the neurobiological basis of psychiatric disease has accelerated in the past five years. The fourth edition of Neurobiology of Mental Illness has been completely revamped given these advances and discoveries on the neurobiologic foundations of psychiatry. Like its predecessors the book begins with an overview of the basic science. The emerging technologies in Section 2 have been extensively redone to match the progress in the field including new chapters on the applications of stem cells, optogenetics, and image guided stimulation to our understanding and treatment of psychiatric disorders. Sections' 3 through 8 pertain to the major psychiatric syndromes-the psychoses, mood disorders, anxiety disorders, substance use disorders, dementias, and disorders of childhood-onset. Each of these sections includes our knowledge of their etiology, pathophysiology, and treatment. The final section discusses special topic areas including the neurobiology of sleep, resilience, social attachment, aggression, personality disorders and eating disorders. In all, there are 32 new chapters in this volume including unique insights on DSM-5, the Research Domain Criteria (RDoC) from NIMH, and a perspective on the continuing challenges of diagnosis given what we know of the brain and the mechanisms pertaining to mental illness. This book provides information from numerous levels of analysis including molecular biology and genetics, cellular physiology, neuroanatomy, neuropharmacology, epidemiology, and behavior. In doing so it translates information from the basic laboratory to the clinical laboratory and finally to clinical treatment. No other book distills the basic science and underpinnings of mental disorders and explains the clinical significance to the scope and breadth of this classic text. The result is an excellent and cutting-edge resource for psychiatric residents, psychiatric researchers and doctoral students in neurochemistry and the neurosciences.

Author: Cord Brakebusch
Publisher: Springer Science & Business Media
ISBN: 9400707509
Category : Medical
Languages : en
Pages : 165

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Book Description
Cell culture based research is important for our understanding of biological processes at the cellular and molecular level. Using this approach, the previous decades have produced a wealth of mechanistic information in all areas of biomedical research. Such in vitro research, however, lacks the complexity of in vivo investigations, where many different cell types interact with each other in a normal, three-dimensional environment, with normal levels of cytokines and growth factors. Furthermore, complex human diseases, such as cancer, diabetes or chronic inflammation, can only be modeled in vivo. Due to its small size, its short reproduction time, and the possibility to introduce specific gene mutations, the mouse has become the favourite mammalian model organism to study in vivo function of genes during development and in disease. This book combines review articles on selected subjects presented at the symposium “Mouse as a Model Organism – From Animals to Cells”, held in Rovaniemi, Finland, 2009. Among other topics, high-throughput phenotyping of mouse mutants, mouse phenotypes dependent on nature and nuture, and a spectrum of in vivo, ex vivo and in vitro methods to study cancer in mice are described. This book will give an excellent introduction to scientists interested in the use of mice as a model to understand complex biological questions in the post-genomic era. It will highlight the possibilities, but also discuss the current problems and shortcomings, to give a realistic view of the current state-of-art in this fascinating field of biomedical research.