Genetically Engineered Mice Handbook PDF Download

Author: John P. Sundberg
Publisher:
ISBN: 9780429122507
Category : Transgenic mice
Languages : en
Pages : 314

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Author: Carl A. Pinkert
Publisher: Newnes
ISBN: 0124095364
Category : Science
Languages : en
Pages : 714

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Book Description
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date

Author: John P. Sundberg
Publisher: CRC Press
ISBN: 1420039075
Category : Medical
Languages : en
Pages : 336

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Book Description
While mice have always been highly popular laboratory subjects, their suitability for genetic engineering has solidified their position as today's lab animal model of choice. However, their increased use in genetic studies has created a demand for input on phenotyping that is not always easily met. To improve the flow of information on the pathology of mice with spontaneous or genetically engineered mutations, prominent researchers organized a series of meetings. Recognizing other needs, the organizers gradually broadened their focus, until finally they expanded to provide an overview of the entire field of genetically engineered models. The Genetically Engineered Mice Handbook is an extension of those meetings. It offers an introduction for those entering into this area of research, while also serving as a resource for those presently employing mice as laboratory models. Highly comprehensive, this volume covers pertinent aspects of genetically engineered mice, including the use of models for developmental biology and the monitoring of laboratory colonies. With contributions from nearly five-dozen leading researchers, the text presents systematic approaches for analyzing mutant mice for specific medical applications, details a variety of methods for creating mutants and includes information that is particularly hard to access dealing with legal responsibilities. This essential reference examines commonly used traditional, as well as emerging, technologies To address the purpose of the original meeting, the Genetically Engineered Mice Handbook directs researchers to the best public websites, and offers instruction on how to use them. In the past, as their work dictated, researchers would seek out experts on particular organ systems. Now groups of experts work together to generate these websites, providing the latest data as well as discussions over points of debate. These sites do not eliminate the need for a trained pathologist, but they do provide reference materials for those lacking expertise in particular anatomic structures. They also offer much greater numbers of examples than are available in print, from which biomedical researchers can draw.

Author: Maurice L. Greensmith
Publisher: CRC Press
ISBN:
Category : Medical
Languages : en
Pages : 566

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Book Description
This second edition of a text for students specializing in laboratory animal science expands coverage to include material on genetic engineering, phenotype characterization of genetically engineered mice, surgical techniques, monitoring the well-being of animals, and the treatment of pain and suffering in laboratory animals. There is discussion of general methods including experimental design and statistical evaluations, non-surgical and surgical techniques, anesthesia, and post-mortem examinations. Safety and security in facilities is examined, and Australasian legislation and regulation are covered. The book is also useful as a reference for biomedical researchers, managers, quality assurance professionals, and technicians. Hau teaches comparative medicine at the University of Uppsala, Sweden; Van Hoosier, at the University of Washington. Annotation copyrighted by Book News, Inc., Portland, OR.

Author: Jann Hau
Publisher:
ISBN:
Category : Animal experimentation
Languages : en
Pages :

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Book Description

Author: Jann Hau
Publisher:
ISBN:
Category : Animal experimentation
Languages : en
Pages :

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Book Description
This second edition of a text for students specializing in laboratory animal science expands coverage to include material on genetic engineering, phenotype characterization of genetically engineered mice, surgical techniques, monitoring the well-being of animals, and the treatment of pain and suffering in laboratory animals. There is discussion of general methods including experimental design and statistical evaluations, non-surgical and surgical techniques, anesthesia, and post-mortem examinations. Safety and security in facilities is examined, and Australasian legislation and regulation are covered. The book is also useful as a reference for biomedical researchers, managers, quality assurance professionals, and technicians. Hau teaches comparative medicine at the University of Uppsala, Sweden; Van Hoosier, at the University of Washington. Annotation copyrighted by Book News, Inc., Portland, OR.

Author: Jann Hau
Publisher: CRC Press
ISBN: 9781420084566
Category : Medical
Languages : en
Pages : 740

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Book Description
Laboratory animal testing provides most of our current knowledge of human physiology, microbiology, immunology, pharmacology, and pathology. From studies of genetics in fruit flies to studies of cellular processes in genetically modified mice to recent dramatic developments in genetics, translational research, and personalized medicines, biomedical

Author: Brian D. Hoit
Publisher: Springer
ISBN: 9781461356615
Category : Science
Languages : en
Pages : 403

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Book Description
The enormous advances in molecular biology and genetics coupled with the progress in instrumentation and surgical techniques have produced a voluminous and often bewildering quantity of data. The need for a second edition of Cardiovascular Physiology in the Genetically Engineered Mouse is underscored not only by these rapid advances, but by the increasing numbers of scientists who have focussed their research on genetically engineered mice. It is the primary objective of this second edition to interpret critically the literature and to provide a framework for the enormous amount of information in this burgeoning field. As in the first edition, the monograph serves as a practical guide for the investigator interested in the functional methods used to characterize the murine cardiovascular phenotype. However, this guidebook is a more comprehensive text than its predecessor; although the major objectives enumerated in the first edition have not substantially changed, they have been refined in keeping with the increased sophistication of the molecular biologist, geneticist, and physiologist in each other's discipline. Each chapter has been expanded and updated, richly enhanced with original tables and figures, and in many cases, extensively rewritten. Eight chapters written by internationally recognized experts have been added; this represents a 43 % increase from the first edition.

Author: Qingbo Xu
Publisher: Wiley
ISBN: 0470028785
Category : Science
Languages : en
Pages : 402

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Book Description
The availability of well-defined genetic strains and the ability to create transgenic and knockout mice makes mouse models extremely valuable biomedical tools. Their suitability as an experimental system for cardiovascular research depends on the individual investigator’s ability to manipulate the mice surgically. Many mouse models require microsurgical techniques, which hitherto could not be performed without practical training. This comprehensive handbook enables scientists to develop these models in their own laboratories. A Handbook of Mouse Models of Cardiovascular Disease is the first book to address pathology in mouse models of heart disease, providing the reader with essential information on technical assays in artificially created models. It includes background information on individual cardiovascular diseases, describes detailed methods and materials used for establishing each mouse model, discusses the problems that may appear in the experiments, and provides examples of applications of the model. A Handbook of Mouse Models of Cardiovascular Disease: Describes mouse models of all important cardiovascular diseases, including atherosclerosis, atrial fibrillation and thrombosis Features videos of key experimental procedures on the accompanying CD, allowing researchers to learn the techniques by directly watching the whole operational procedure Describes how to establish each experimental model with detailed protocols and tips on dealing with common operational problems Highlights potential applications of each model in areas such as pathogenesis, gene transfer, therapy and pathophysiology This handbook is an invaluable resource for researchers in cardiovascular disease, pathology, physiology, interested in the mechanism of vascular disorders and therapeutic approaches. It is also relevant to clinicians seeking to understand the pathology of cardiovascular disease and the rationale for interventions, and of interest to the pharmaceutical industry and all those involved in drug discovery/development for cardiovascular disease.

Author: John P. Sundberg
Publisher: John Wiley & Sons
ISBN: 1119624576
Category : Medical
Languages : en
Pages : 496

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Book Description
An updated and comprehensive reference to pathology in every organ system in genetically modified mice The newly revised and thoroughly updated Second Edition of Pathology of Genetically Engineered and Other Mutant Mice delivers a comprehensive resource for pathologists and biomedical scientists tasked with identifying and understanding pathologic changes in genetically modified mice. The book is organized by body system, includes descriptions and explanations of a wide range of findings, as well as hundreds of color photographs illustrating both common and rare lesions that may be found in genetically engineered and wild type mice. The book is written by experienced veterinary and medical pathologists working in veterinary medical colleges, medical colleges, and research institutes. Covering the latest discoveries in mouse pathology resulting from advancements in biotechnology research over the last 30 years, this singular and accessible resource is a must-read for veterinary and medical pathologists and researchers working with genetically engineered and other mice. Readers will also benefit from: A thorough introduction to mouse pathology and mouse genetic nomenclature, as well as databases useful for analysis of mutant mice An exploration of concepts related to validating animal models, including the Cinderella Effect Practical discussions of basic necropsy methods and grading lesions for computational analyses Concise diagnostic approaches to the respiratory tract, the oral cavity and GI tract, the cardiovascular system, the liver and pancreas, the skeletal system, and other tissues As a one-stop and up to date reference on mouse pathology, Pathology of Genetically Engineered and Other Mutant Mice is an essential book for veterinary and medical pathologists, as well as for scientists, researchers, and toxicologists whose work brings them into contact with genetically modified mice.